Monoterpenes – ABC CARDIOL

ABC

Original Article

(-)-Carvone Modulates Intracellular Calcium Signaling with Antiarrhythmic Action in Rat Hearts

Gilmara Beatriz Andrade da Silva et al. Diego Santos Souza José Evaldo Rodrigues Menezes-Filho Júlio Alves da Silva-Neto … Jader dos Santos Cruz Danilo R. Roman-Campos Lucindo José Quintans-Júnior Carla Maria Lins de Vasconcelos

Abstract

Background:

(-)-Carvone is a monoterpene found in essential oils with antioxidant and anti-inflammatory activity.

Objective:

The aim of this paper was to analyze the antiarrhythmic property of (-)-carvone in the rat heart and its effects on the intracellular Ca²⁺ signaling.

Methods:

The effects of (-)-carvone were evaluated on the ventricular (0.5 mM) and atrial contractility (0.01 – 4 mM) and on electrocardiogram (0.5 mM). Fractional shortening, L-type calcium current (I_Ca,L) and Ca²⁺ signaling were measured in the isolated cardiomyocyte (0.5 mM). Antiarrhythmic effect was evaluated in arrhythmia model induced by calcium overload (0.5 mM) (n = 5). P < 0.05 was used as the significance level.

Results:

In the atrium, (-)-carvone evoked negative inotropism that was concentration-dependent (EC50 0.44 ± 0.11 mM) and decreased the positive inotropism evoked by CaCl₂ (0.1 to 8.0 mM) or BAY K8644 (5 to 500 nM), an agonist of L-type Ca²⁺ channel. In isolated heart, (-)-carvone (0.5 mM) promoted reduction of ventricular contractility (73%) and heart rate (46%), increased PRi (30.7%, time from the onset of the P wave until the R wave) and QTc (9.2%, a measure of the depolarization and repolarization of the ventricles) without changing the QRS complex duration. (-)-Carvone decreased the fractional shortening (61%), I_Ca,L (79%) and Ca²⁺ intracellular transient (38%). Furthermore, (-)-carvone showed antiarrhythmic action, verified by decrease of the arrhythmia score (85%) and occurrence of ventricular fibrillation.

Conclusion:

(-)-Carvone decreases Ca²⁺ entry through L-type Ca²⁺ channels, reducing the cardiac contractility and intracellular Ca²⁺, and, therefore, presenting promising antiarrhythmic activity in the rat hearts.

Read Full Article

ABC

Editorial

D-Limonene: A Promising Molecule with Bradycardic and Antiarrhythmic Potential

Quiara Lovatti Alves et al. Darizy Flávia Silva

It is unquestionable that cardiovascular disease (CVD) is and will continue to be one of the main health problems of modern society. A significant portion of cardiovascular disease-related deaths is a direct or indirect consequence of cardiac arrhythmias.Cardiac arrhythmias are abnormal conditions that refer to any alteration in the normal sequence of cardiac electrical impulses, which can be classified according to frequency, mechanism, duration, and place of origin, resulting in inefficient pumping. More specifically, cardiac arrhythmias indicate alterations in the rate or regularity of the heart rhythm, as well as changes in the conduction sequence of physiological electrical impulses, from their origin in the sinoatrial node to their end, in the His-Purkinje system, and the ventricles.The structural mechanisms that result in cardiac arrhythmias, as well as limitations regarding the safety and efficacy of “classic” antiarrhythmic drugs, make this disease difficult to treat.

The use of medicinal plants for the treatment, cure and prevention of diseases is one of the oldest forms of human medicinal practice. Natural medicinal plant-based products have shown to be an abundant source of biologically active compounds, many of which have been the basis for the development of new chemical products by the pharmaceutical industry.Essential oils, extracted from aromatic plants, are natural products with a variety of biological actions, including cardiovascular properties.Monoterpenes are the most representative molecules, which constitute 90% of essential oils, and also produce relevant effects on measurable cardiovascular parameters, including vasorelaxation and hypotension promotion in anesthetized and non-anesthetized rats.^, Additionally, monoterpenes may attenuate the development of hypertension in spontaneously hypertensive rats, by decreasing cardiac hypertrophy. Thus, it is evident that monoterpenes are molecules that could be further developed for the prevention and / or treatment of cardiovascular diseases. A study developed by our research group showed that the monoterpene carvacrol has negative chronotropic and inotropic effects on rats, whereas pulegone and geraniol have a negative inotropic effect on mammals.^,

[…]

Read Full Article

ABC

Original Article

Bradycardic and Antiarrhythmic Effects of the D-Limonene in Rats

Gildenisson Araújo do Nascimento et al. Diego Santos de Souza Bruno Santos Lima Carla Maria Lins de Vasconcelos … Adriano Antunes de Souza Araújo Aimée Obolari Durço Lucindo José Quintans-Junior Jackson Roberto Guedes da Silva Almeida Aldeída Pereira Oliveira Valter Joviniano de Santana-Filho André Sales Barreto Márcio Roberto Viana dos Santos

Abstract

Background:

D-limonene (DL) is a monoterpene and is the major component in the essential oil of citrus fruit. It presents antihyperglycemic and vasodilatation activities.

Objectives:

This study evaluated the cardiovascular effects and potential antiarrhythmic of DL in rats.

Methods:

Hemodynamic and electrocardiographic (ECG) parameters were measured in male Wistar rats, which under anesthesia had been cannulated in the abdominal aorta and lower vena cava and had electrodes subcutaneously implanted. In the in vitro approach, the heart was removed and perfused using the Langendorff technique. The significance level adopted was 5% (p < 0.05).

Results:

DL, in doses of 10, 20, and 40 mg/kg (i.v), produced intense and persistent bradycardia associated with hypotension. Bradycardia with prolonged QTc was observed in the ECG in vivo recording. In the in vivo model of arrhythmia induced by Bay K8644, DL (10 mg/kg) decreased the arrhythmia score from 15.33 ± 3.52 to 4.0 ± 2.64 u.a (p < 0.05, n = 4). In isolated perfused hearts, DL (10^-3 M) promoted significant reductions in heart rate (from 228.6 ± 8.5 ms to 196.0 ± 9.3 bpm; p < 0.05) and left ventricular development pressure (from 25.2 ± 3.4 to 5.9 ± 1.8 mmHg; n = 5, p < 0.05).

Conclusions:

DL produces bradycardia and antiarrhythmic activity in rat heart.

Read Full Article

(-)-Carvone Modulates Intracellular Calcium Signaling with Antiarrhythmic Action in Rat Hearts

Abstract

Background:

Objective:

Methods:

Results:

Conclusion:

D-Limonene: A Promising Molecule with Bradycardic and Antiarrhythmic Potential

Bradycardic and Antiarrhythmic Effects of the D-Limonene in Rats

Abstract

Background:

Objectives:

Methods:

Results:

Conclusions:

Electronic Document Format (Vancouver)

Electronic Document Format (ABNT)

Electronic Document Format (APA)

Electronic Document Format (ISO)

Electronic Document Format (Vancouver)

Electronic Document Format (ABNT)

Electronic Document Format (APA)

Electronic Document Format (ISO)

Electronic Document Format (Vancouver)

Electronic Document Format (ABNT)

Electronic Document Format (APA)

Electronic Document Format (ISO)