Leonardo Mateus Teixeira de Rezende et al. Leôncio Lopes Soares Filipe Rios Drummond Pedro Zavagli Suarez … Luciano Leite Joel Alves Rodrigues Tiago Leal Lukiya Favarato Emily Correna Carlo Reis Evandro Favarato Miguel Carneiro Júnior Antônio José Natali Cândido Coimbra Thales Prímola Gomes
Abstract
Background:
There are divergences in the literature regarding the experimental model (Wistar-WIS or Wistar Kyoto-WKY) to be used as a Spontaneously Hypertensive Rat (SHR) control. The characterization of these models in terms of cardiovascular parameters provides researchers with important tools at the time of selection and application in scientific research.
Objective:
The aim of this study was to evaluate the use of WIS and WKY as a Spontaneously Hypertensive Rat (SHR) control by assessing the long-term behavior of blood pressure and cardiac structure and function in these strains.
Methods:
To this end, WIS, WKY, and SHR underwent longitudinal experiments. Blood pressure and body mass were measured every two weeks from the 8th to the 72nd. Echocardiographic analysis was performed in all groups with 16, 48, and 72 weeks of life. After having applied the normality test, the Two-Way ANOVA of repeated measures followed by the Tukey post hoc test was used. A significance level of 5% was established.
Results:
The WIS group showed higher body mass (p<0.05), while the WKY and SHR presented higher body mass variation over time (p<0.05). SHR exhibited increased values of systolic, diastolic, and mean blood pressure when compared to WKY and WIS, whereas the WKY generally showed higher values than WIS (p<0.05). Regarding the cardiac function, SHR showed reduced values, while the WKY presented an early decrease when compared to WIS with aging (p<0.05).
Conclusion:
WIS is a more suitable normotensive control for SHR than WKY in experiments to test blood pressure and cardiac structure and function.